Crass-like phage

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August undefined, 2024

WebFeb 16, 2024 · Some of the crAss-like phage genomes (for example, Eld241-t0_s_1 of the delta group; Supplementary Fig. 7) contain almost no long ORFs when translated under … WebHowever, crAss-like phages, in this population, are dominated by the genera VI, VII and VIII. Interestingly, it also reveals the presence of a virus family, Sphaerolipoviridae, which has not been ...

Acquisition, transmission and strain diversity of human gut …

WebAnother crAss-like phage, V016904, is shown in Supplementary Figure 5. Display full size. Figure 4. Multiomic sequencing map of V014624, a crAss-like phage identified in the colonic mucosal-luminal interface. Open reading frames (ORFs) were predicted over the 97.9 kb genome and colored by forward (green) or reverse (purple) orientation ... WebOct 23, 2024 · Two of the 39 phage genomes — crAss-like phage and LoVEphage — showed remarkably high prevalence worldwide, at 20.6% and 14.4%, respectively. The researchers identified one as a crAss-like ... fore river parkway portland maine

GitHub - gbouras13/pharokka: fast phage annotation program

WebJan 16, 2024 · CrAss-like phage genomes assemble very easily out of metagenomic sequencing data, and the small genome size makes measuring strain diversity simple. While many methods have been developed to characterize strain diversity in bacteria, such as StrainPhlAn[12] PanPhlAn[13], our relatively simple read mapping approach worked … WebHere, using Cellulophaga baltica crAss-like phage phi14:2 as a model system, we show that this protein is a DNA-dependent RNAP that is translocated into the host cell along with the phage DNA and transcribes early phage genes. We determined the crystal structure of this 2,180-residue enzyme in a self-inhibited state, which probably occurs ... WebNov 14, 2024 · CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic … fore river hospital portland maine

The crAss-like Phage Group: How Metagenomics Reshaped

Large-scale phage cultivation for commensal human gut bacteria

WebThe first members of the crAss-like group was recently isolated and shown to infect the bacterium Bacteroides intestinales. Characterization of this phage validated the predicted podovirus-like virion structure and the identity of the major capsid protein and other predicted virion proteins, including three RNA polymerase subunits. WebJun 1, 2024 · Assembly and identification of crAss-like phage genomes To identify crAss-like phage genomes in our gut metagenomic data, we assembled contigs for 2291 metagenomic samples from four cohorts: 1135 LLD, 338 LLD follow-up, 298 300OB and 520 IBD samples. Contigs of 10 kilobases (kb) or longer were retained for further analysis. fore river management portland maineWebApr 12, 2024 · By mapping metagenomic reads to the phage genomes (coverage ≥ 30%), we discovered that the prevalence of 2 GPIC phages (Parabacteroide phage PD491P1 29.5%, and Alistipes phage AS73P1 19.5%) was higher than that of the two isolated crAss-like phages (crAss001, 11.3% and crAss002, 14.5%) but lower than that of the prototype … did zoro die from taking all of luffy\\u0027s pain

"WebFeb 28, 2024 · crAss-like phages possess unusual features of genome organization, for example, genes encoding giant proteins consisting of fused RNA polymerase subunits. The first crAss-like phage has been isolated and shown to infect Bacteroides intestinalis. Metagenomics is currently the primary means for identifying new viruses. " - Crass-like phage

Crass-like phage

Discovery, diversity and functional associations of …

WebJul 5, 2024 · CrAss-like phages that resolve into five of the ten crAss-like family candidate genera were detected. c Absolute quantification of each crAss-like phage detected using qPCR with phage or genus ... WebFeb 26, 2024 · The crAss-like phages are therefore reminiscent of the concept of plasmid or phage plasmid (Lagos and Goldstein, 1987). Finally, coupling metaHiC with other …

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The crAss-like phage bacteriophage family is considered highly diverse and consists of four subfamilies- alpha, beta, delta, and gamma- and ten genera within the subfamilies. The subfamilies are defined by crAss-like phage that share 20-40% of their protein-encoding genes while a genera is … See more CrAss-like phage are a bacteriophage (virus that infects bacteria) family that was discovered in 2014 by cross assembling reads in human fecal metagenomes. In silico comparative genomics and taxonomic analysis have … See more The crAss (cross-assembly) software used to discover the first crAss-like phage, p-crAssphage (prototypical-crAssphage), relies on cross assembling reads from multiple metagenomes obtained from the same environment. The goal of cross-assembly is that … See more CrAss-like phage have been identified as a highly abundant and near-universal member of the human gut microbiome. CrAss-like phage … See more • Newly-found gut virus 'abundant in humans' - BBC News • Why Has This Really Common Virus Only Just Been Discovered? - National Geographic See more The crAss-like phage have a podoviridae-like morphology with a tail structure similar to that of bacteriophage P22. Based on initial sequence-based studies of crAss-like phage, the bacteriophage family was predicted to consist of phage with a diversity of … See more Based on a sequence similarity screen of p-crAssphage protein sequences to protein sequences in public sequence databases and … See more

WebJun 1, 2024 · Assembly and identification of crAss-like phage genomes To identify crAss-like phage genomes in our gut metagenomic data, we assembled contigs for 2291 … WebNov 29, 2024 · Some of the most striking phage counter-resistance mechanisms include alteration of phage DNA to evade host restriction modification mechanisms ( 63) and phage encoded CRISPR-Cas systems that target and disable a range of host defense systems ( 64 ). Ecological Relevance of Phage-Host Dynamics

WebJan 11, 2024 · The crAss-like phage taxonomic clusters involved in these associations included the entire crAss-like phage assemblage, groups beta and delta, and genus-level clusters alpha15, gamma1, gamma4, delta27, and epsilon1. All the bacteria involved in these associations belonged to the phyla Actinobacteria and Firmicutes. WebJan 15, 2024 · CrAss-like phages are double-stranded DNA viruses that are prevalent in human gut microbiomes. Here, we analyze gut metagenomic data from mother-infant pairs and patients undergoing fecal...

WebAug 18, 2024 · The crAss-like phages are ubiquitous and highly abundant members of the human gut virome that infect commensal bacteria of the order Bacteroidales. Although incapable of lysogeny, these viruses demonstrate long-term persistence in the human gut microbiome, dominating the virome in some individuals. Results

WebApr 12, 2024 · The first crAss-like phage isolated, ΦcrAss001, infects B. intestinalis and is a member of the more heterogenous candidate genus VI of the sub-family … did zoro die when he took luffy\\u0027s painWebFeb 26, 2024 · The crAss-like phages are therefore reminiscent of the concept of plasmid or phage plasmid (Lagos and Goldstein, 1987). Finally, coupling metaHiC with other approaches on larger human cohort will shed new lights and findings about the relationships between phages, plasmids, and bacteria. did zora neale hurston have siblingsWebJul 20, 2024 · Conserved and group-specific genomic features in the extended crAss-like phage assemblage Examination of the TerL tree suggests a coarse-grain structure of the relationships among the crAss-like phages, which include the 596-strong human gut ‘crAss-virome’, the previously analyzed environmental contigs and several … did zoro sleep with hiyoriWebAug 18, 2024 · CrAss-like phages are capable of long-term stable persistence in the mammalian gut and select for resistance in their bacterial host. We have previously … did zora neale hurston have a husbandWebMay 22, 2024 · A new crAss-like genome encoding diversity-generating retroelement (DGR) was found in the fecal virome of a healthy volunteer. The genome of the phage referred to as the crAssphage LMMB, belonged to the candidate genus I of the AlphacrAssvirinae subfamily. The DGR-cassette of the crAssphage LMMB contained all … did zoro die from taking all of luffy\u0027s painWebFeb 1, 2024 · Their genome sizes (Phage TG-crAlp-04 -100,626 bp, Phage TG-crAlp-06 -98,173 bp) fell in the general size range of crAss-like phages which are typically around 100 kb in size (Guerin et al., 2024). did zuckerberg say posting the lord\u0027s prayerWebNov 13, 2024 · Some crAss-like phages encode additional, auxiliary proteins in the tail module, for example, an IAS protein homologous to the tail-associated lysozyme gp13 of … fore river parkway portland me