Total erk antibody
WebProduct Specific Information. Description: The 5AD13MA monoclonal antibody recognizes human, mouse, and rat extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/42, or MAPK3/1). ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) that are involved in ... WebNormally in my laboratory, I use an antibody which detects Total Erk (both: the phosphorylated and the non-phosphorylated kinase) as a loading control for western blot, in apoptosis studies.
Total erk antibody
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WebProduct Specific Information. Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody … WebERK 1 Primary Antibody for Size Assays Erk 1 Rabbit Primary Antibody, 1X stock (1 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. 042-488 HeLa Lysate Controls HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. 043-311 Luminol-S
WebActivation of Erk (extracellular signal regulated kinase) requires the phosphorylation of Thr202 and Tyr204 amino acid residues. NIH/3T3 cell pellets (Figure A) treated with either … WebSanta Cruz Biotechnology, Inc. offers a broad range of ERK antibodies. Select ERK antibodies from monoclonal antibodies listed below. View detailed ERK antibody specifications by linking to the specific product blocks. Select appropriate ERK antibodies for your research by isotype, epitope, applications and species reactivity.
WebSep 28, 2024 · This antibody was used to detect total ERK expression in prostate cell line PNT2, PC3 and DU145. The data showed ERK can be detected in all of three cell lines. … Webone 96-well assay with the phospho-ERK antibody and one 96-well assay with the total-ERK antibody. 9.1 1X PBS: Prepare 1X PBS by adding 1 volume of 10X PBS (pH 7.4) to 9 volumes of dH2O and mixing thoroughly. 9.2 Preparation of Fixing Buffer (4% or 8% Formaldehyde in PBS): Fixing Buffer is used to fix cells after cell culturing. Prepare by
WebHRP conjugated antibodies were used as the secondary antibodies. Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) …
WebApr 11, 2024 · Quantification of the relative normalized levels of Ncl antibody signal in the Ctrl vs. Myc-Ncl expressing cells from (A). Myc-Ncl transfected cells exhibit total Ncl levels that are around 50% more than those of the Ctrl cells. A total of three independent biological replicates per condition were analyzed. how to create a histogram in rstudioWebOct 22, 2024 · (C) Levels of p-Erk, Erk and bax in the control group, vehicle group, anti-TNF-α antibody group and anti-TNF-α antibody+PMA group were detected by Western blotting. ( … how to create a histogram in rWeb7. Dilute the primary antibody in TBST to the recommended dilution. We recommend incubating in a sealed bag, hybridization tube or 50 ml Falcon tubes (~2.5 ml primary antibody/blot). Incubate overnight at 4°C with agitation. 8. Rinse the blot in TBST three to four times for 5 min each at room temperature. 9. how to create a histogram in stataWebThe cells were labeled with ERK1 + ERK2 Antibody [ERK-7D8] (ab54230) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 … microsoft office money in excelWebMay 20, 2024 · ERK 2 Antibody (D-2) is an IgG2b κ mouse monoclonal MAPK1 antibody that detects m, r, and h ERK 2 p42 by WB, IP, IF, IHC(P), FCM and ELISA. 570 citations. Choose … microsoft office monthly planWebAug 31, 2024 · The proteins were extracted and subjected to SDS-PAGE and Western blotting analysis. Anti-phosphorylated ERK and total ERK antibodies were used to analyze ERK protein. The fold changes were indicated under the phosphorylated and total ERK bands relative to the GFP siRNA baselines. C-H, DENV2 titers is increased in the Ras knockdown … microsoft office monthly costWebSee: MAPK/ERK pathway. Transgenic gene knockout mice lacking MAPK1 have major defects in early development. Conditional deletion of Mapk1 in B cells showed a role for MAPK1 in T-cell-dependent antibody production. A dominant gain-of-function mutant of Mapk1 in transgenic mice showed a role for MAPK1 in T-cell development. how to create a histogram in word